Journal: Small (Weinheim an der Bergstrasse, Germany)

Article Title: Liposome-Polymer Nanoparticles Loaded with Copper Diethyldithiocarbamate and 6-Bromo-Indirubin-3'-Oxime Enable the Treatment of Refractive Melanoma.

doi: 10.1002/smll.202409012

Figure Lengend Snippet: Figure 6. A) Difference in cytokine levels in isolated serum from B16F10 mice treated with 3 mg/kg of vehicle (n = 4), LPBIO (n = 5), LPCuET (n = 5), and LPBC (n = 4) at 24h post IV injection showing a notable decrease in inflammatory cytokines and chemokines when treated with LBPC compared to LPCuET, One-Way ANOVA with Dunnett’s correction was used, empty circles represent individual mice and data is shown as mean. B) Heat map showing the difference in the expression of select cytokine and chemokine mRNA extracted from whole ectopic B16F10 tumors of mice treated with the various groups showing a reduction of pro-inflammatory markers when BIO is used alone or in combination with CuET, data depicted as the geometric mean of the fold change in gene expression (2-ΔΔCt) from vehicle-treated mice. C) Representative lung pictures and lesion count in B16F10 lung metastases showing the extent of the tumor burden in mice injected with control B16F10 cells compared to B16F10 injected alongside 3 mg/kg LPBC, or B16F10 cells pre-treated with 1 and 5 μm of LPBC for 3h before IV injection, showing that the exposure of cells to LPBC before injection into mice can diminish their ability to form metastases, black nodules represent individual tumors. D,E) Differential fold change in the gene expression profile of surface receptors relevant for NK cell recognition in D) B16F10 cells and E) A375 cells, empty circles represent individual experiments, and data is shown as geometric mean ± SD. F,G) Flow cytometry experiment showing the increase in the proportion of F) NKp44L+ B16F10 cells and G) ULBP1+ A375 cells when treated with LPBC, student’s t-test with Welch’s correction was used, and data is shown as mean. H,I) Flow cytometry experiment showing the proportion and mean fluorescence intensity (MFI) of H) PD1+ and I) CD69+ Jurkat cells when activated in the presence of BIO, CuET, or the combination compared to vehicle control, Welsch’s One-Way ANOVA with Dunnett’s 3T correction was used, empty circles represent individual experiments and data is shown as mean.

Article Snippet: Monoclonal antibodies αPD1 (RMP1-14), αCTLA4 (9D9), and isotype IgG2a (2A3) and IgG2b (MPC-11) control antibodies, and 6.5 pH dilution buffer (IP0065) were purchased from Bio X Cell (Lebanon, NH).

Techniques: Isolation, IV Injection, Expressing, Gene Expression, Injection, Control, Flow Cytometry